RNA
Part:BBa_K4687047:Design
Designed by: Yiming Jiang Group: iGEM23_HBUT-China (2023-10-12)
sgRNA2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The editing of the target gene was achieved by the binding of sgRNA to Cas proteins and its import into cells.
Source
The final sgRNA was obtained by synthesis and ligation of the two partial sequences.