RNA

Part:BBa_K4687047:Design

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-12)


sgRNA2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The editing of the target gene was achieved by the binding of sgRNA to Cas proteins and its import into cells.


Source

The final sgRNA was obtained by synthesis and ligation of the two partial sequences.

References